2,6-Dimethyl-4-quinolinecarboxylic acid N-hydroxysuccinimide ester: A fluorogenic hydrophilic derivatizing reagent for liquid chromatographic analysis of aliphatic amines
✍ Scribed by Hong Wang; Xin Liu; Niu Zhang; Li-Wei Cao; Hua-Shan Zhang
- Publisher
- John Wiley and Sons
- Year
- 2006
- Tongue
- English
- Weight
- 464 KB
- Volume
- 29
- Category
- Article
- ISSN
- 1615-9306
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✦ Synopsis
Paper 2,6-Dimethyl-4-quinolinecarboxylic acid N-hydroxysuccinimide ester: A fluorogenic hydrophilic derivatizing reagent for liquid chromatographic analysis of aliphatic amines
The use of a fluorogenic, hydrophilic, and amine-reactive reagent, 2,6-dimethyl-4quinolinecarboxylic acid N-hydroxysuccinimide ester (DMQC-OSu) has been investigated in the procolumn derivatization for the LC separation of aliphatic amines. In pH 8.0 aqueous medium, DMQC-OSu reacted with amines at 508C within 20 min to form highly fluorescent carboxamides and the excess reagent hydrolyzed to the corresponding carboxylic acid. The separation of representative amine derivatives with DMQC-OSu has been performed using a C 18 column with the fluorescence detection at 326/409 nm. The detection limits reached nanomolar level. The proposed method has been applied to the analysis of real samples with recoveries of 94 -108%. Compared with other succinimidyl esters used in the derivatization of amino compounds, DMQC-OSu and its hydrolysate had negligible fluorescence (b fl = 0.09 and 0.02, respectively), which implied that small peaks appeared in chromatograms and slight interference was introduced to the determination.