2,4-Dinitrobenzenesulfonyl Fluoresceins as Fluorescent Alternatives to Ellman's Reagent in Thiol-Quantification Enzyme Assays

2,4-Dinitrobenzenesulfonyl Fluoresceins

✍ Hatsuo Maeda; Hiromi Matsuno; Mai Ushida; Kohei Katayama; Kanako Saeki; Norio It 📂 Article 📅 2005 🏛 John Wiley and Sons 🌐 English ⚖ 388 KB

Since its first description in 1959, [1] 5,5'-dithio-bis(2-nitrobenzoic acid) (DTNB or Ellmans reagent) has been used widely for the quantification of thiols, especially for assays of various enzymes including acetyl-and butyrylcholinesterase (AChE and BChE, respectively) [2] in which substrates rel