2006 Abstracts: Twenty-Eighth Annual Meeting of the American Society for Bone and Mineral Research: Pennsylvania Convention Convention Center Philadelphia, Pennsylvania, USA, September 15–19, 2006
- Publisher
- American Society for Bone and Mineral Research
- Year
- 2006
- Tongue
- English
- Weight
- 977 KB
- Volume
- 21
- Category
- Article
- ISSN
- 0884-0431
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✦ Synopsis
Recent efforts to enhance craniofacial reconstruction using bone allografts have focused on the adjunctive clinical benefits of growth and amelogenin-like factors. The purpose of this study was to evaluate the effects of enamel matrix derivative (EMO) and rhPOGF, in combination with freeze-dried or demineralized bone matrix (OBM), on mineralization rate in an osteotomy defect model. Critical size calvarial defects (7 mm) were created in 60 Wistar male rats (350 g). Animals were randomly assigned to treatment with human freeze-dried or OBM ($4 % calcium) alone or in combination with EMO or rhPOGF (n = 10 per group). At 28 days post-grafting, animals were sacrificed and calvarial specimens were fixed in 10% neutral formalin and submitted to microradiographic analysis. Radiodensitometric assessment of the defects was performed using computer assisted image analysis. Radiodensity scores were submitted to ANCOVA, controlling for bone density proximal to the defect. The results revealed differential effects of EMO and rhPOGF on rate of mineralization (radiodensity) which were dependent on the graft matrix (Table ; Adjusted Means ± SO). Defects grafted with freeze-dried bone exhibited a significantly higher rate of mineralization when combined with either EMO or rhPOGF. In contrast, OBM exhibited similar levels of mineralization, whether alone or in combination with a biologic mediator. The application of both EMO and rhPOGF significantly enhanced the rate of mineralization in critical-sized defects grafted with freeze-dried (i.e., nondemineralized) bone matrix, when compared to graft alone, resulting in radiodensity levels comparable to those obtained with OBM. In contrast, the mineralization rate of defects grafted with DBM exhibited no demonstrable change in rate of mineralization in response to EMD or rhPDGF.
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