2-D Proteome Analysis Protocols Volume 112 || Sample Preparation Methods for Mass Spectrometric Peptide Mapping Directly from 2-DE Gels
✍ Scribed by Link, Andrew J.
- Book ID
- 111987092
- Publisher
- Humana Press
- Year
- 1998
- Tongue
- English
- Weight
- 210 KB
- Category
- Article
- ISBN-13
- 9781592595846
No coin nor oath required. For personal study only.
✦ Synopsis
A Step-by-step Tour Through The Complete Process Of Doing Proteomics. With Easy-to-follow Instructions, Complete With Many Helpful Hints And Explanations, Leading Investigators And Pioneers In The Field Show How To Make Protein Extracts, Reproducibly Run Them On 2-d Gels, Detect Them, Analyze The Data, And Precisley Identify Each Protein. The Book Covers The Latest Methods Of Using Carrier Ampholytes In The 1st Dimension, Casting And Running Immobilized Ph Gradient 2-d Gels, Maldi-tof-based Peptide Mapping, Automated Tandem Mass Spectrometry, And Nanoelectrospray Ionization Technology. For The Second Dimension, There Are Methods For Running Flatbed Or Vertical Gels And For Protein Detection Using Autoradiography, And Coomassie, Silver, And Reversible Metal-chelate Stains. 2-d Proteome Analysis Protocols Is The Most Complete Guide For Using Proteomics To Answer Biological Questions.