2-(6,7-Dimethoxy-2,3-Naphthalimido)ethyl Trifluoromethanesulfonate as a Labeling Reagent for Carboxylic Acids in Liquid Chromatography

✍ Scribed by M. Tanaka; H. Muramatsu; Y. Yasaka

• Book ID: 102615221
• Publisher: Elsevier Science
• Year: 1994
• Tongue: English
• Weight: 246 KB
• Volume: 49
• Category: Article
• ISSN: 0026-265X
• DOI: 10.1006/mchj.1994.1024

✦ Synopsis

A new, highly reactive and sensitive labeling reagent, 2-(6,7-dimethoxy-2,3naphthalimido)ethyl trifluoromethanesulfonate, (6,7-DMNE-OTf), has been synthesized and used to determine several carboxylic acids by high-performance liquid chromatography. Reactions of the reagent with long-chain, saturated monocarboxylic acids proceed to completion within (5 \mathrm{~min}) in acetone at room temperature in the presence of 18 -crown-6 as a catalyst. The acid derivatives could be readily eluted with acetonitrile-water ( (90: 10) ). The detection limits (signal-to-noise ratio (=3) ) with ultraviolet and fluorescence detection are 10 (\mathrm{fmol}\left(\lambda_{\max }=282 \mathrm{~nm}\right)) and (3 \mathrm{fmol}\left(\lambda_{\mathrm{ex}}=282 \mathrm{~nm}, \lambda_{\mathrm{em}}=456 \mathrm{~nm}\right)), respectively. (1994 Academic Press, Inc.