1α,25-Dihydroxyvitamin D3 modulation in lipid metabolism in established bone marrow-derived stromal cells, MC3T3-G2/PA6
✍ Scribed by Manabu Shionome; Toshimasa Shinki; Naoyuki Takahashi; Kohji Hasegawa; Dr. Tatsuo Suda
- Book ID
- 102878660
- Publisher
- John Wiley and Sons
- Year
- 1992
- Tongue
- English
- Weight
- 709 KB
- Volume
- 48
- Category
- Article
- ISSN
- 0730-2312
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✦ Synopsis
Abstract
MC3T3‐G2/PA6 (PA6) cells established from newborn mouse calvaria are preadipocytic stromal cells, which differentiate into adipocytes in response to glucocorticoids. We examined the effects of 1α,25‐dihydroxyvitamin D~3~[1α,25(OH)~2~D~3~] on adipogenesis in PA6 cells were cultured with 10^−8^ M dexamethasone, adipocytes containing oil red O‐positive droplets first appeared on day 7 (3 days after confluence was attained) and the maximal synthesis of neutral lipids occurred on day 12. Simultaneous addition of 1α,25(OH)~2~D~3~ at 10^−9^ M completely blocked this dexamethasone‐induced neutral lipid synthesis throughout the 14‐day culture period. Dose‐response studies of vitamin D~3~ derivatives showed that 1α,25(OH)~2~D~3~ was the most potent in inhibiting neutral lipid synthesis in PA6 cells, followed by 1α‐hydroxyvitamin D~3~, 25‐hydroxyvitamin D~3~ and 24R,25‐dihydroxyvitamin D~3~, in that order. Dexamethasone greatly enhanced incorporation of [^14^C]‐acetic acid into triacylglycerol in PA6 cells. The incorporation was markedly inhibited by the addition of 10^−9^ M 1α,25(OH)~2~D~3~ Instead, 1α,25(QH)~2~D~3~ greatly increased incorporation of [^14^C]‐acetic acid into phospholipids, such as phosphatidylcholine and phosphatidylethanolamine, irrespective of the presence or absence of dexamethasone. These results suggest that 1α,25(OH)~2~D~3~ modulation of lipid metabolism in bone marrow stromal cells is receptor mediated.