1H relaxation times of metabolites in biological samples obtained with nondestructive ex-vivo slow-MAS NMR
✍ Scribed by Jian Zhi Hu; Robert A. Wind; Donald N. Rommereim
- Publisher
- John Wiley and Sons
- Year
- 2006
- Tongue
- English
- Weight
- 165 KB
- Volume
- 44
- Category
- Article
- ISSN
- 0749-1581
- DOI
- 10.1002/mrc.1764
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✦ Synopsis
Abstract
Methods suitable for measuring ^1^H relaxation times such as T~1~, T~2~ and T~1ρ~ of metabolites in small, intact biological objects including live cells, excised organs and tissues, oil seeds etc. are developed in this work. This was achieved by combining inversion‐recovery, spin‐echo, or a spin‐lock segment with the phase‐adjusted spinning sideband (PASS) technique, which was applied at low sample‐spinning rates. Here, PASS was used to produce high‐resolution ^1^H spectra in a nondestructive way so that the relaxation parameters of individual metabolite could be determined. The methodologies were demonstrated by measuring ^1^H T~1~, T~2~, and T~1ρ~ of metabolites in excised rat liver at a spinning rate of 40 Hz. Copyright © 2006 John Wiley & Sons, Ltd.