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1H-NMR assay of phosphatidylcholine and phosphatidylethanolamine in AL721

โœ Scribed by Andrew P. Cheung; Lori L. Olson

Publisher
Elsevier Science
Year
1990
Tongue
English
Weight
301 KB
Volume
8
Category
Article
ISSN
0731-7085

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โœฆ Synopsis

A 1H-NMR based method has been developed for the assay of phosphatidylcholine (PC), and phosphatidylethanolamine (PE) in AL721. The assay was based on the comparison of NMR signal intensities of the choline and the ethanolamine moiety of PC and PE, respectively, to the signal of imidazole, the internal standard. The assay was reliable and more accurate than the commonly used LC or TLC methods. The uncertainty, due primarily to signal intensities, of the 1H-NMR method was estimated at less than 3%.

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