Ion trap MS has been utilized in the structure elucidation of a saponin from Quillaja saponaria. With respect to the carbohydrate moieties, this technique allowed the elucidation of the sequence and branching, of six out of seven glycosidic linkages, the precise location of an appended O-acetyl grou
1H NMR as a Tool for the Structure Elucidation of Moenomycin Antibiotics
β Scribed by Astrid Donnerstag; Lothar Hennig; Matthias Findeisen; Peter Welzel; Rainer Haessner
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 351 KB
- Volume
- 34
- Category
- Article
- ISSN
- 0749-1581
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β¦ Synopsis
As a consequence of aggregate formation, no 'H NMR spectra of the amphiphilic antibiotic moenomycin A have been obtained previously. It was now found that the system moenomycin A-D20-C,D, after sonification yielded highly resolved 'H NMR spectra, probably due to the formation of a microemulsion. Similarly resolved spectra were obtained from moenomycin A in sodium dodecyl sulphate micelles.
π SIMILAR VOLUMES
High-resolution 'H NMR and molecular mechanics calculations were used to evaluate the solution structure of TRH (pyroglutamic acid-histidine-prolinamide). The results indicate that there are only two rotamers present at room temperature with a sufficient energy barrier on the 400 MHz NMR time-scale