1H NMR Analysis for Metabolites in Serum and Urine from Rats Administrated Chronically with La(NO3)3
✍ Scribed by Jianghua Feng; Xiaojing Li; Fengkui Pei; Xi Chen; Shulei Li; Yuxiu Nie
- Book ID
- 102561190
- Publisher
- Elsevier Science
- Year
- 2002
- Tongue
- English
- Weight
- 84 KB
- Volume
- 301
- Category
- Article
- ISSN
- 0003-2697
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✦ Synopsis
has been used to assess longterm toxicological effects of a rare earth. Male Wistar rats were administrated orally with La(NO 3 ) 3 at doses of 0.1, 0.2, 2.0, 10, and 20 mg/kg body wt, resp., for 3-6 months. Urine was collected at 1, 2, and 3 months and serum samples were taken after 6 months. Numerous low-M r metabolites in rats serum and rats urine, including creatinine, citrate, glucose, ketone bodies, trimethylamine N-oxide (TMAO), and various amino acids, were identified on 400-and 500-MHz 1 H NMR spectra. La 3؉ -induced renal and liver damage is characterized by an increase in the amounts of the excreted ketone bodies, amino acids, lactate, ethanol, succinate, TMAO, dimethylamine, and taurine and a decrease in citrate, glucose, urea, and allantoin. Information on the molecular basis of the long-term toxicity of La(NO 3 ) 3 was derived from the abnormal patterns of metabolite excretions. An assay of some biochemical indexes and analysis of some enzymes in plasma supported NMR results.