1H HR-MAS spectroscopy for quantitative measurement of choline concentration in amniotic fluid as a marker of fetal lung maturity: Inter- and intraobserver reproducibility study
✍ Scribed by Bonnie N. Joe; Kiarash Vahidi; Andrew Zektzer; Mei-Hsiu Chen; Matthew S. Clifton; Thomas Butler; Kayvan Keshari; John Kurhanewicz; Fergus Coakley; Mark G. Swanson
- Publisher
- John Wiley and Sons
- Year
- 2008
- Tongue
- English
- Weight
- 401 KB
- Volume
- 28
- Category
- Article
- ISSN
- 1053-1807
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
Purpose
To determine the intra‐ and interobserver reproducibility of human amniotic fluid metabolite concentration measurements (including potential markers of fetal lung maturity) detectable by MR spectroscopy.
Materials and Methods
^1^H high‐resolution magic angle spinning (HR‐MAS) spectroscopy was performed at 11.7T on 23 third‐trimester amniotic fluid samples. Samples were analyzed quantitatively using 3‐(trimethylsilyl)propionic‐2,2,3,3‐d~4~ acid (TSP) as a reference. Four observers independently quantified eight metabolite regions (TSP, lactate doublet and quartet, alanine, citrate, creatinine, choline, and glucose) twice from anonymized, randomized spectra using a semiautomated software program.
Results
Excellent inter‐ and intraobserver reproducibility was found for all metabolites. Intraclass correlation as a measure of interobserver agreement for the four readers ranged from 0.654 to 0.995. A high correlation of 0.973 was seen for choline in particular, a major component of surfactant. Pearson correlation as a measure of intraobserver reproducibility ranged from 0.478 to 0.999.
Conclusion
Quantification of choline and other metabolite concentrations in amniotic fluid by high‐resolution MR spectroscopy can be performed with high inter‐ and intraobserver reproducibility. Demonstration of reproducible metabolite concentration measurements is a critical first step in the search for biomarkers of fetal lung maturity. J. Magn. Reson. Imaging 2008;28:1540–1545. © 2008 Wiley‐Liss, Inc.