1H 2D-NMR characterization of Ni(II)-substituted azurin from Pseudomonas aeruginosa

✍ Scribed by José-María Moratal; Jesús Salgado; Antonio Donaire; Hermas R. Jiménez; Josep Castells; María-josé Martínez-ferrer

• Publisher: John Wiley and Sons
• Year: 1993
• Tongue: English
• Weight: 576 KB
• Volume: 31
• Category: Article
• ISSN: 0749-1581
• DOI: 10.1002/mrc.1260311310

✦ Synopsis

Abstract

^1^H two‐dimensional NMR experiments on nickel(II)‐substituted azurin have been successfully applied. Despite the short relaxation times of the hyperfine‐shifted resonances, the combined use of NOESY and COSY spectra allowed the assignment of 15 resonances belonging to the metal‐coordinated residues Gly‐45, His‐46, His‐117 and Met‐121. Even in the case of the two broad and furthest downfield resonances, the NOESY spectra were successful in assigning these signals to the β‐CH~2~ protons of Cys‐112. The protons of the non‐coordinated residues Met‐13, Phe‐15 and Trp‐48 were also assigned via NOESY, COSY and TOCSY experiments.