1H-, 13C-, and 113Cd-nmr study of the Cd(II) complex of a blocked peptide, Z-Cys-Ala-Pro-His-OMe, in organic solvents
✍ Scribed by Hiroaki Zaima; Norikazu Ueyama; Hayamitsu Adachi; Akira Nakamura
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 1995
- Tongue
- English
- Weight
- 826 KB
- Volume
- 35
- Category
- Article
- ISSN
- 0006-3525
No coin nor oath required. For personal study only.
✦ Synopsis
H-, I3C-, and 113 Cd-NMR Study of the Cd( 11) Complex of a Blocked Peptide, Z-Cys-Ala-Pro-His-OMe, in Organic Solvents
The Cd(II) complex ofa peptide, Z-Cys-Ala-Pro-His-OMe was prepared and characterized by absorption, CD, 'H-, I3C-, and ""Cd-nmr, and nirclear Overhazrser effect spectroscopy (NOESY) spectra to show the coordination of cysteine thiolate and histidine imizazole to Cd(II) ion. The NOESY spectra in dimethy1,formamide showed that the cysteine residue was in proximity to the histidine residue. These results reveal the chelation of Z-Cys-Ala-Pro-His-OMe to Cd(I1) ion in solution. Temperature-dependent dissociation eqiiilibrium of histidine imidazole in solution was observed in this complex. Striictural featiires ofthe chelating peptide are discussed. 0 I995 John Wi1e.v & Sons, Inc.