18 O-Labeling of N-Glycosylation Sites To Improve the Identification of Gel-Separated Glycoproteins Using Peptide Mass Mapping and Database Searching

A method for determination of N-glycosyl

A method for determination of N-glycosylation sites in glycoproteins by collision-induced dissociation analysis in fast atom bombardment mass spectrometry: Identification of the positions of carbohydrate-linked asparagine in recombinant α-amylase by treatment with peptide-N-glycosidase F in 18O-labeled water

✍ Javier Gonzalez; Toshifumi Takao; Hideaki Hori; Vladimir Besada; Rolando Rodrigu 📂 Article 📅 1992 🏛 Elsevier Science 🌐 English ⚖ 1006 KB

Previously, a combined use of fast atom bombardment (FAB) mass spectrometry and peptide N-glycosidase F, an enzyme that cleaves the beta-aspartylglycosylamine linkage of Asn-linked carbohydrates, was successfully applied to identification of N-glycosylation sites in a glycoprotein with the known or