17α, 20α-dihydroxy-4-pregnen-3-one, not its 20β isomer, is produced from 17α-hydroxyprogesterone by spermatozoa of secondary male groupers (Epinephelus tauvina) derived from females implanted with 17α-methyltestosterone
✍ Scribed by Tan, A. M. C. ;Lee, S. T. L. ;Kime, D. E. ;Chao, T. M. ;Lim, H. S. ;Chou, R. ;Lam, T. J. ;Tan, C. H.
- Publisher
- John Wiley and Sons
- Year
- 1995
- Tongue
- English
- Weight
- 366 KB
- Volume
- 271
- Category
- Article
- ISSN
- 0022-104X
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✦ Synopsis
Sperm of secondary male groupers (Epinephelus tauuina), derived from females implanted with 17a-methyltestosterone (MTj, were incubated with [3H117-hydroxyprogesterone for 2 hr. The medium was extracted and analyzed by high performance liquid chromatography (HPLC). A single major metabolite with relative retention time corresponding to 17a,20adihydroxy-4-pregnen-3-one (17,201~-P) was produced by the sperm. The metabolite was isopolar with 17,201~-P on thin layer chromatography (TLCj. The presumptive identity of the metabolite was confirmed to be 17,20a-P when the metabolite was oxidised by periodic acid and the derivative recrystallized with authentic androstenedione to specific constant activity. This experiment indicates that grouper sperm possess the enzyme, 20a-hydroxysteroid dehydrogenase (20a-HSD1, and suggests that 17,20a-P may play a role in grouper reproduction. 0 1995 wiley-~iss, Inc.