17-β-Estradiol upregulates the stress response in Candida albicans: Implications for microbial virulence
✍ Scribed by C. O'Connor; M. Essmann; B. Larsen
- Book ID
- 101262905
- Publisher
- Hindawi Publishing Corporation
- Year
- 1998
- Tongue
- English
- Weight
- 241 KB
- Volume
- 6
- Category
- Article
- ISSN
- 1064-7449
No coin nor oath required. For personal study only.
✦ Synopsis
Objective: The influence of 17--estradiol on the stress response of Candida albicans was studied.
Methods: The survival of clinical isolates of C. albicans treated with 17--estradiol after heat and oxidative stress was measured by viable plate counts. Cellular proteins were analyzed via SDS-PAGE.
Results: The heat stress response induced by 17--estradiol in C. albicans grown at 25°C protected the organisms against the lethal temperature of 48.5°C, as shown by viable plate counts. 17--estradiol also enhanced protection of C. albicans against oxidative stress (menadione exposure). SDS-PAGE analysis of cytoplasmic extracts revealed proteins induced by 17--estradiol were similar to those induced by heat.
Conclusion: 17--estradiol enhances survival of C. albicans under heat and oxidative stresses. The proteins induced by 17--estradiol are probably heat shock proteins. Because heat shock proteins are considered to be virulence factors, 17--estradiol may function to promote in vivo survival.