17?-estradiol attenuates CREB decline in the rat hippocampus following seizure

✍ Scribed by Panickar, Kiran S. ;Guan, Guangwei ;King, Michael A. ;Rajakumar, Gopal ;Simpkins, James W.

• Publisher: John Wiley and Sons
• Year: 1997
• Tongue: English
• Weight: 269 KB
• Volume: 33
• Category: Article
• ISSN: 0022-3034
• DOI: 10.1002/(sici)1097-4695(199712)33:7<961::aid-neu7>3.0.co;2-6

✦ Synopsis

Cyclic AMP response element-bindafter recovery and the brains were processed for CREB ing protein (CREB) is a transcription factor that has immunoreactivity. CREB-positive neurons were been implicated in the activation of a number of genes. counted using a computer-assisted program. Insulin We reported that CREB levels decline following a severe treatment of OVX rats caused a significant decline in hypoglycemic episode in the hippocampus and cortex in CREB-positive neurons in the CA1, CA3, and dentate the male rat brain. The present experiment was undergyrus compared to OVX rats. Estrogen treatment of taken to investigate whether 17b-estradiol prevents the OVX rats significantly increased CREB-positive neudecline in CREB-immunoreactive cells following seizure rons in the CA1 and dentate gyrus and attenuated the in female rats. Rats were divided into four groups: ovariinsulin-induced decline of CREB-positive neurons in all ectomized (OVX), ovariectomized and insulin-treated three regions compared to OVX rats. In conclusion, es-(OVX-I), estrogen-replaced (E2), and estrogen-retrogens appear to induce CREB expression and attenuplaced and insulin-treated (E2-I). Generalized seizures ate its decline in the hippocampus following a severe were induced by injections with insulin (12.5 IU/kg, hypoglycemic episode. ᭧ 1997 John Wiley & Sons, Inc. J intraperitoneally) and animals were recovered by ad-Neurobiol 33: 961-967, 1997 ministration of glucose within 5 min of the occurrence