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15N,1H coupling constants in pyridines and pyrimidines An application of the INEPT pulse sequence

✍ Scribed by W. Städeli; P. Bigler; W. Von Philipsborn


Publisher
John Wiley and Sons
Year
1981
Tongue
English
Weight
282 KB
Volume
16
Category
Article
ISSN
0749-1581

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✦ Synopsis


Abstract

Application of a modified INEPT pulse sequence to obtain fully proton‐coupled and selectively decoupled ^15^N NMR spectra of pyridines and pyrimidines with natural isotope abundance is demonstrated. The spectra are analysed for ^2^J, ^3^J and ^4^J(NH) coupling constants.


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A set of three improved two-dimensional (2D) NMR methods for measuring one-bond 15 N-1 H coupling constants in the protein backbone is presented. They are tailored to suit the size of the TROSY effect, i.e., the degree of interference between dipolar and chemical shift anisotropy relaxation mechanis