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154-P LinkSeq™ and SureTyper™ – a rapid real-time PCR system for HLA typing

✍ Scribed by Zachary C. Antovich; Roland Russnak; Ngoc Ly; Kai Quinto; Matthew Frome; Eric Mitchell; F. Carl Grumet

Book ID
116533331
Publisher
Elsevier Science
Year
2011
Tongue
English
Weight
41 KB
Volume
72
Category
Article
ISSN
0198-8859

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## Abstract We describe the use of quantitative PCR (QPCR) to titer recombinant baculoviruses. Custom primers and probe were designed to gp64 and used to calculate a standard curve of QPCR derived titers from dilutions of a previously titrated baculovirus stock. Each dilution was titrated by both p