1,4-Dioxane is not mutagenic in five in vitro assays and mouse peripheral blood micronucleus assay, but is in mouse liver micronucleus assay
✍ Scribed by Takeshi Morita; Makoto Hayashi
- Publisher
- John Wiley and Sons
- Year
- 1998
- Tongue
- English
- Weight
- 166 KB
- Volume
- 32
- Category
- Article
- ISSN
- 0893-6692
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✦ Synopsis
1,4-Dioxane, an animal carcinogen, was not pre-concentration we used was five mg/ml or plate. viously genotoxic in in vitro assays. We reevaluated We also evaluated the genotoxic effect of 1,4-dioxthe compound's genotoxic potential in five in vitro ane in vivo by conducting peripheral blood and genotoxicity tests in the presence and absence of liver micronucleus assays in the same mice after single S9 mix using recommended new protocols. We oral administration of up to 3,000 mg/kg. All in vitro used the bacterial reverse mutation assay with Sal-assays and the peripheral blood micronucleus assay monella TA and E. coli WP2 strains, including the were negative. The mouse liver micronucleus assay, plate and preincubation methods, the CHO chromo-on the other hand, was positive, indicating that 1,4somal aberration assay, including examination of dioxane might be genotoxic. It is also conceivable that polyploid induction and extended sampling time, the positive result in mouse liver micronucleus assay the CHO sister-chromatid exchange assay with was due to a nongenotoxic mechanism, i.e., errors in short and long treatment time, the mouse lymphoma genetic repair following enhancement of hepatocyte tk assay (microtiter method), including longer treat-proliferation. Environ. Mol. Mutagen. 32:269-280, ment time (24 hr), and the CHO micronucleus assay 1998 ᭧ 1998 Wiley-Liss, Inc. with short and long treatment times. The highest Key words: 1