13C nuclear spin-spin relaxation times (T2Cs) of enantiomers in the presence of column packing material and solvents for chiral discrimination HPLC
✍ Scribed by Kazuma Oguni; Masaaki Ito; Akira Isokawa; Akiko Matsumoto
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 466 KB
- Volume
- 8
- Category
- Article
- ISSN
- 0899-0042
No coin nor oath required. For personal study only.
✦ Synopsis
To elucidate chiral recognition difference between pairs of enantiomers which interacted with a cellulosic stationary phase of chiral discrimination high performance liquid chromatography (HPLC), 13C NMR spectroscopy was applied for enantiomers of 1phenylethanol, 1-indanol, 1,2,3,4,-tetrahydro-l-naphthol, glutethimide, and Troger's base.
Their 13C nuclear spin-spin relaxation times (Tzcs) and spectra were obtained in deuterated HPLC solvents in the presence of column packing material made of cellulose tris(4methylbenzoate) coated on silica gel. The first-eluted enantiomers on the HPLC showed longer Tzcs and stronger signal intensities of 13C NMR spectra than the second-eluted enantiomers. These results indicated that the chiral recognition difference of enantiomers was observed by their Tzcs and intensities of 13C NMR spectra. The Tzc difference was found to reflect the retention order of enantiomers on the chiral HPLC column.