13C NMR studies of fatty acid-protein interactions: comparison of homologous fatty acid-binding proteins produced in the intestinal epithelium
β Scribed by David P. Cistola; James C. Sacchettini; Jeffrey I. Gordon
- Publisher
- Springer
- Year
- 1990
- Tongue
- English
- Weight
- 729 KB
- Volume
- 98
- Category
- Article
- ISSN
- 0300-8177
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π SIMILAR VOLUMES
Sequence-specific 1H, 13C and 15N resonance assignments have been established for rat intestinal fatty acid-binding protein complexed with palmitate (15.4 kDa) at pH 7.2 and 37 degrees C. The resonance assignment strategy involved the concerted use of seven 3D triple-resonance experiments (CC-TOCSY,
## Abstract In a previous study we had demonstrated that a 15βkDa protein present in carp intestinal brushβborder membrane vesicles (BBMV) was able to bind the endocytosis tracer horseradish peroxidase (HRP) with high specificity. Here we show that this protein corresponds to a peripheral membrane