13C NMR kinetic studies of the rapid stimulation of glucose metabolism by estrogen in immature rat uterus

✍ Scribed by Leonid Shinkarenko; Alvin M. Kaye; Hadassa Degani

• Publisher: John Wiley and Sons
• Year: 1994
• Tongue: English
• Weight: 894 KB
• Volume: 7
• Category: Article
• ISSN: 0952-3480
• DOI: 10.1002/nbm.1940070503

✦ Synopsis

Glucose metabolism in control and estrogen stimulated rat uteri was investigated using "C NMR spectroscopy. By employing an NMR adapted perifusion system, and developing protocols and methods based on the application of [l-'3C]glucose labeling, it was possible to measure, with a temporal resolution of 10 min, the kinetics of glucose consumption, lactate production and "C incorporation into glutamate, alanine and glycogen.

In control immature rat uteri, under aerobic conditions, the rates (+SEM) of [l-''C]glucose consumption and (J-''C]lactate production were 24f2 and 7.5f0.5mmollg wet weightlh. The rates of synthesis of [4-"C]glutamate, [3-''C]alanine and "C labeling of glycogen at Cl of its glucose moieties were significantly lower and were in the range 0.3-0.6 mmol/g wet weightlh. Thus, ca 35% of the labeled glucose was accounted for by the glycolytic and other observed pathways. I n uifro stimulation of the uteri by estrogen was found to increase significantly, within 1 h, glucose consumption by SO%, lactate production by 150% and glutamate and glycogen synthesis by 150%, in parallel to a rapid hormonal induction of mRNA for the brain type isozyme of creatine kinase.