1,25-dihydroxy vitamin D3 and 12-O-tetradecanoyl phorbol-13-acetate synergistically induce monocytic cell differentiation: FOS and RB expression

✍ Scribed by Andrew Yen; Mark Coles; Susi Varvayanis

• Book ID: 102883838
• Publisher: John Wiley and Sons
• Year: 1993
• Tongue: English
• Weight: 637 KB
• Volume: 156
• Category: Article
• ISSN: 0021-9541
• DOI: 10.1002/jcp.1041560126

✦ Synopsis

Abstract

1,25‐dihydroxy vitamin D3 and 12‐O‐tetradecanoyl phorbol‐13‐acetate (TPA) interact synergistically to induce monocytic differentiation of U937 histiocytic lymphoma cells. Addition of TPA causes an otherwise ineffective dose of 1,25‐dihydroxy vitamin D3 to induce differentiation. The induced differentiation depends on the simultaneous (vs. sequential) presence of both agents. The kinetics of induced differentiation are consistent with a G1 specific cellular response to initiate the metabolic cascade culminating in cell differentiation. The induced differentiation occurs with down‐regulation of c‐fos protein and an accompanying up‐regulation of RB protein expression, consistent with a possible need for up‐regulated RB expression to maintain a given differentiated phenotype and suppress transcriptional activators that might typically be associated with proliferation. © 1993 Wiley‐Liss, Inc.