12-Hydroxy-5Z, 8Z, 10E, 14Z, eicosatetraenoic acid (12-HETE) stimulates cAMP production in normal human fibroblasts

✍ Scribed by Shoko Ueki; Junichi Takagi; Yuichi Kobayashi; Fumie Sato; Yuji Saito

• Publisher: John Wiley and Sons
• Year: 1999
• Tongue: English
• Weight: 150 KB
• Volume: 178
• Category: Article
• ISSN: 0021-9541
• DOI: 10.1002/(sici)1097-4652(199901)178:1<63::aid-jcp8>3.0.co;2-j

✦ Synopsis

We report here that the 12-lipoxygenase metabolite of arachidonic acid, 12hydroxy-5Z, 8Z, 10E, 14Z, eicosatetraenoic acid (12-HETE), stimulates cAMP production in human fibroblasts among various cultured cell lines tested. Although 12-HETE seemed to stimulate the phospholipase C (PLC)-protein kinase C (PKC) system, inhibitors against PLC and PKC did not reduce the cAMP production induced by 12-HETE, indicating that the activation of PLC-PKC system is not positively coupled with the stimulation of cAMP production. On the other hand, the cAMP production induced by 12-HETE was dependent on the Ca 2ϩ /calmodulin system in the cells. The results suggest that 12-HETE specifically stimulates Ca 2ϩ /calmodulin-dependent adenylyl cyclase to increase cAMP level in the fibroblasts.