113Cd NMR spectra of human serum transferrin

✍ Scribed by William Kiang; Peter J. Sadler; David G. Reid

• Publisher: John Wiley and Sons
• Year: 1993
• Tongue: English
• Weight: 286 KB
• Volume: 31
• Category: Article
• ISSN: 0749-1581
• DOI: 10.1002/mrc.1260311321

✦ Synopsis

Abstract

The binding of Cd^2+^ to human serum transferrin (80 kDa) was studied by ^113^Cd NMR spectroscopy. At pH 7 in the presence of hydrogen‐carbonate as synergistic anion, two resonances of similar intensity at 38.1 and 43.6 ppm were observed, consistent with Cd^2+^ binding to oxygen ligands and one nitrogen ligand in the specific metal‐binding clefts of the N‐ and C‐lobes of the protein.