1,1,1,3,3,3-hexafluoroisopropanol induced thermal unfolding and molten globule state of bovine α-lactalbumin: Calorimetric and spectroscopic studies
✍ Scribed by Agnita Kundu; Nand Kishore
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 2004
- Tongue
- English
- Weight
- 204 KB
- Volume
- 73
- Category
- Article
- ISSN
- 0006-3525
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✦ Synopsis
Abstract
The thermal denaturation of α‐lactalbumin was studied at pH 7.0 and 9.0 in aqueous 1,1,1,3,3,3‐hexafluoroisopropanol (HFIP) by high‐sensitivity differential scanning calorimetry. The conformation of the protein was analyzed by a combination of fluorescence and circular dichroism measurements. The most obvious effect of HFIP was lowering of the transition temperature with an increase in the concentration of the alcohol up to 0.30__M__, beyond which no calorimetric transition was observed. Up to 0.30__M__ HFIP the calorimetric and van't Hoff enthalpy remained the same, indicating the validity of the two‐state approximation for the thermal unfolding of α‐lactalbumin. The quantitative thermodynamic parameters accompanying the thermal transitions have been evaluated. Spectroscopic observations confirm that α‐lactalbumin is in the molten globule state in the presence of 0.50__M__ HFIP at pH 7.0 and 0.75__M__ HFIP at pH 9.0. The results also demonstrate that α‐lactalbumin in the molten globule state undergoes a noncooperative thermal transition to the denatured state. It is observed that two of four tryptophans are exposed to the solvent in the HFIP induced molten globule state of α‐lactalbumin compared to four in the 8.5__M__ urea induced denatured state of the protein. It is also observed that the HFIP induced molten globule states at the two pH values are different from the acid induced molten globule state (A state) of α‐lactalbumin. © 2004 Wiley Periodicals, Inc. Biopolymers, 2004