1-Hydroxypyrene in human urine after exposure to coal tar and a coal tar derived product

✍ Scribed by F. J. Jongeneelen; R. B. M. Anzion; Ch.-M. Leijdekkers; R. P. Bos; P. Th. Henderson

• Publisher: Springer-Verlag
• Year: 1985
• Tongue: English
• Weight: 460 KB
• Volume: 57
• Category: Article
• ISSN: 0340-0131
• DOI: 10.1007/bf00383545

✦ Synopsis

A method for isolating 1-hydroxypyrene from urine is described. The presence of 1-hydroxypyrene in urine was identified by fluorescence excitation and emission scanning after HPLC-separation 1-Hydroxypyrene could be detected in the urine of rats following oral administration of as little as 0 5 g pyrene The dose-dependence of 1-hydroxypyrene in urine was evident after a wide range of pyrene dosing After therapeutical coal tar treatment of dermatological patients the enhanced excretion of 1-hydroxypyrene was highly significant Employees of a creosote impregnating plant showed an excretion pattern of 1-hydroxypyrene which could be related to their work 1-Hydroxypyrene in urine of non-exposed people was very low, but detectable It is suggested that the method reported is suitable for the assessment of uptake of man to pyrene, a compound that is commonly present in work environments which are associated with pollution of polycyclic aromatic hydrocarbons.